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Home > Vol 34, No 4 (2024) > Muanakewjinda

Preparation and Characterization of Antibacterial Hydrogels from Poly(vinyl alcohol)/Aloe vera/Chitosan for Burn Wound Dressing Application

Chanoksuda Muanakewjinda, Nadda Wanghitang, Tanwalai Chatkumpjunjalearn, Wilaiporn Kraisuwan, Pakjirat Singhaboot

Abstract


ไคโตซานเป็นพอลิแซ็กคาไรด์ธรรมชาติที่ได้จากไคติน ไม่มีความเป็นพิษ เข้ากันได้ทางชีวภาพ ย่อยสลายได้ทางชีวภาพ มีสมบัติในการห้ามเลือด และยับยั้งเชื้อแบคทีเรีย ทำให้สามารถประยุกต์ใช้งานด้านชีวการแพทย์ได้หลากหลาย ในงานวิจัยนี้ได้เตรียมไฮโดรเจลของพอลิไวนิลแอลกอฮอล์ ว่านหางจระเข้ และไคโตซาน โดยการผสมสารละลายทั้งหมดเข้าด้วยกันจากนั้นเชื่อมขวางด้วยกลูตารัลดีไฮด์ เพื่อประยุกต์ใช้เป็นวัสดุปิดแผลไฟไหม้ โดยได้ศึกษาปริมาณของไคโตซาน (1% 2% และ 3%) และกลูตารัลดีไฮด์ (2.5% 5% และ 10%) ที่ส่งผลต่อสมบัติทางกายภาพ สมบัติเชิงกล และฤทธิ์ต้านแบคทีเรียของไฮโดรเจลที่เตรียมได้ จากนั้นวิเคราะห์โครงสร้างของไฮโดรเจลด้วยเทคนิคฟูเรียร์ทรานฟอร์มอินฟราเรดสเปกโทรสโกปี ผลการวิจัยพบว่า สัดส่วนการบวมตัว อัตราการซึมผ่านไอน้ำ การยุบตัว และความพรุนของไฮโดรเจลเพิ่มขึ้นตามปริมาณของไคโตซานที่เพิ่มขึ้น แต่ลดลงเมื่อปริมาณของกลูตารัลดีไฮด์เพิ่มขึ้น นอกจากนี้การเพิ่มปริมาณของไคโตซาน และกลูตารัลดีไฮด์ยังส่งผลให้ความต้านทานแรงดึงมีแนวโน้มเพิ่มขึ้นเล็กน้อย ในขณะที่ความยืดสูงสุด ณ จุดขาดมีแนวโน้มลดลง การประเมินสมบัติการยับยั้งเชื้อแบคทีเรีย แสดงให้เห็นว่าไฮโดรเจลสามารถยับยั้งได้ทั้งเชื้อแบคทีเรียแกรมบวก (S. aureus) และแบคทีเรียแกรมลบ (E. coli) ผลการวิจัยนี้แสดงให้เห็นว่า ปริมาณของไคโตซานและกลูตารัลดีไฮด์เป็นปัจจัยสำคัญในการเตรียมวัสดุปิดแผลเพื่อให้ได้วัสดุที่มีสมบัติเหมาะสมสำหรับใช้ปิดแผล สรุปได้ว่า ไฮโดรเจลของพอลิไวนิลแอลกอฮอล์ว่านหางจระเข้ และไคโตซาน มีศักยภาพในการพัฒนาต่อไปเป็นวัสดุปิดแผลไฟไหม้ในอุดมคติ

Chitosan is a natural polysaccharide derived from chitin. It has non-toxic, biocompatible, biodegradable, hemostatic and antibacterial properties that can be used in a wide range of biomedical applications. In this study, the hydrogels of poly(vinyl alcohol), aloe vera and chitosan were prepared by mixing their aqueous solutions followed by crosslinking with glutaraldehyde for usage in burn wound dressing application. The effects of the chitosan (1%, 2% and 3%) and glutaraldehyde contents (2.5%, 5% and 10%) on physical, mechanical properties and antibacterial activities of the hydrogels were studied. The structure of the hydrogels was characterized by using Fourier transform infrared spectroscopy. The results showed that swelling ratio, water vapor transmission rate, deswelling and porosity of the hydrogels increased with the increase of chitosan content but decreased with the increase of glutaraldehyde content. Besides, the increase of chitosan and glutaraldehyde contents resulted in slightly increased tensile strength, while elongation at break decreased. Antibacterial assessment showed that the hydrogels inhibited both Gram-positive bacteria (S. aureus) and Gram-negative bacteria (E. coli). The results indicated that the contents of chitosan and glutaraldehyde are the key factors in obtaining such suitable properties required for the wound dressing. In conclusion, the hydrogels of poly (vinyl alcohol), aloe vera and chitosan have the potential to be further developed as an ideal burn wound dressing material.


Keywords



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DOI: 10.14416/j.kmutnb.2024.08.006

ISSN: 2985-2145