สมบัติการยับยั้งเชื้อแบคทีเรียของอนุภาคคอปเปอร์บนถ่านกัมมันต์ที่เตรียมด้วยการเอิบชุ่มแบบแห้งและแบบเปียก
Antibacterial Performance of Copper Particles Doped on Activated Carbon Prepared by Wet and Dry Impregnation Methods
Abstract
ถ่านกัมมันต์ซึ่งถูกเตรียมจากของเหลือทิ้งทางการเกษตรได้ถูกนำมาใช้บำบัดน้ำกันอย่างกว้างขวาง การเพิ่มสมบัติในการยับยั้งแบคทีเรียของถ่านกัมมันต์ดึงดูดความสนใจของนักวิจัยในการนำไปประยุกต์ใช้ในงานต่าง ๆ งานวิจัยนี้ศึกษาสมบัติการยับยั้งแบคทีเรียของคอปเปอร์บนถ่านกัมมันต์ที่เตรียมจากเม็ดบ๊วย (AAC) และเปรียบเทียบกับถ่านกัมมันต์ทางการค้า (CAC) อนุภาคคอปเปอร์ถูกเตรียมโดยวิธีเอิบชุ่มแบบเปียก (W) และแบบแห้ง (D) ด้วยสารละลายคอปเปอร์ (II) ไนเตรต เพื่อให้ได้ความเข้มข้นของคอปเปอร์ร้อยละ 5 โดยน้ำหนัก ผลวิเคราะห์องค์ประกอบจากเทคนิคการเลี้ยวเบนของรังสีเอ็กซ์พบ Cu0 ในตัวอย่างที่เตรียมด้วยวิธีการเอิบชุ่มแบบเปียก (CAC-5W และ AAC-5W) ส่วน CuO และ Cu2O มักถูกพบในตัวอย่างที่เตรียมด้วยวิธีการเอิบชุ่มแบบแห้ง (CAC-5D และ AAC-5D) ภาพถ่ายจากกล้องจุลทรรศน์อิเล็กตรอนแบบส่องกราดแสดงให้เห็นว่าอนุภาคคอปเปอร์/คอปเปอร์ออกไซด์บน CAC-5W มีกระจายตัวได้ดีกว่าตัวอย่าง CAC-5D จากผลการวิเคราะห์สมบัติการยับยั้งเชื้อแบคทีเรียด้วยวิธี Disc Diffusion พบว่า ตัวอย่างที่เตรียมโดยวิธีเอิบชุ่มแบบเปียก (AAC-5W และ CAC-5W) แสดงสมบัติการยับยั้งเชื้อ Escherichia coli ATCC 25922 และ Staphylococcus aureus ATCC 25923 ได้ดีกว่าตัวอย่างที่เตรียมโดยวิธีการเอิบชุ่มแบบแห้ง (AAC-5D และ CAC-5D) ซึ่งแสดงสมบัติการยับยั้งเชื้อ E. coli เพียงอย่างเดียว ดังนั้นสรุปได้ว่าคอปเปอร์ที่เตรียมโดยวิธีเอิบชุ่มแบบเปียกมีสมบัติการยับยั้งเชื้อแบคทีเรียได้ดีกว่าที่เตรียมโดยวิธีการเอิบชุ่มแบบแห้ง
Activated carbon produced from agricultural wastes has been widely used in water treatment. Enhancing antibacterial properties of activated carbon has attracted researchers’ attention towards many applications. This research aims to study the antibacterial properties of copper impregnated on Apricot stone Activated Carbon (AAC) and compare with those of Commercial Activated Carbon (CAC). The copper particles were prepared by Wet impregnation (W) and Dry impregnation (D) methods with copper (II) nitrate solution to obtain 5%wt of Cu concentration. The XRD results demonstrated that Cu0 was found on activated carbons prepared by the wet impregnation method (CAC-5W and AAC-5W) whereas both CuO and Cu2O were mostly found on activated carbons prepared by the dry impregnation method (CAC-5D and AAC-5D). Furthermore, SEM images showed that the Cu/CuxO particles are well dispersed on CAC-5W than on CAC-5D. From the antibacterial activity evaluated by disc diffusion method showed that AAC-5W and CAC-5W exhibited the inhibition zone against both Escherichia coli ATCC 25922 and Staphylococcus aureus ATCC 25923 whereas AAC-5D and CAC-5D exhibited the inhibition zone against only E. coli. Therefore, it can be concluded that copper loaded on activated carbon prepared by the wet impregnation exhibiting higher antibacterial activity than that by the dry impregnation.
Keywords
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DOI: 10.14416/j.kmutnb.2024.07.007
ISSN: 2985-2145
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