อิทธิพลของปริมาณสารสกัดมะแขว่นต่อลักษณะทางเคมีกายภาพและฤทธิ์ทางชีวภาพของนาโนอิมัลชัน
Effect of Zanthoxylum Limonella (Dennst.) Alston. Content on Physicochemical Properties and Bioactivity of Nanoemulsion
Abstract
งานวิจัยนี้มีวัตถุประสงค์เพื่อศึกษาอิทธิพลของปริมาณสารสกัดมะแขว่นต่อลักษณะทางเคมีกายภาพ ฤทธิ์ทางชีวภาพและความคงตัวของนาโนอิมัลชัน โดยทำการศึกษาปริมาณสารสกัดมะแขว่นที่แตกต่างกันได้แก่ 1% (ZLNE 1%) 5% (ZLNE 5%) และ 10% (ZLNE 10%) จากนั้นทำการวิเคราะห์ขนาดอนุภาค ศักย์ซีต้า สี ประสิทธิภาพการห่อหุ้มสารสำคัญ ทดสอบฤทธิ์ต้านอนุมูลอิสระ วิเคราะห์ปริมาณสารฟีนอลิกและปริมาณสารฟลาโวนอยด์ จากผลการทดลองพบว่า การเพิ่มปริมาณสารสกัดมะแขว่นส่งผลให้นาโนอิมัลชันมีขนาดอนุภาคใหญ่ขึ้นอย่างมีนัยสำคัญ (p < 0.05) ค่าศักย์ซีต้าลดลง ค่าสีเขียวและสีเหลืองเพิ่มขึ้น ค่าความสว่างมีแนวโน้มลดลง สำหรับการศึกษาประสิทธิภาพการห่อหุ้มสาร 4-terpineol พบว่า ZLNE 10% มีร้อยละการห่อหุ้มมากที่สุด คือ 94.73% ซึ่งสอดคล้องกับการทดสอบฤทธิ์ทางชีวภาพที่พบว่า ZLNE 10% มีฤทธิ์ต้านอนุมูลอิสระ ปริมาณสารฟีนอลิก และปริมาณสารฟลาโวนอยด์ มากกว่า ZLNE 5% และ ZLNE 1% อย่างมีนัยสำคัญทางสถิติ (p < 0.05) กล่าวโดยสรุป การเพิ่มปริมาณสารสกัดมะแขว่นส่งผลให้ฤทธิ์ทางชีวภาพของนาโนอิมัลชันเพิ่มมากขึ้น อย่างไรก็ตามยังต้องมีการปรับปรุงตำรับนาโนอิมัลชันให้มีความคงตัวมากยิ่งขึ้นเพื่อให้สามารถนำไปใช้ในผลิตภัณฑ์ยาและเครื่องสำอางได้
The objective of this research was to investigate the effect of Zanthoxylum Limonella (Dennst.) Alston. extract (ZL extract) content on physicochemical properties and bioactivity of nanoemulsion. Nanoemulsions were prepared with various amounts of ZL extract including 1% (1% ZLNE), 5% (5% ZLNE) and 10% (10% ZLNE). Particle size, zeta potential, color, encapsulation efficiency, antioxidant activity, phenolic content, and flavonoid content were evaluated. The results showed that increasing the content of the ZL extract significantly enlarged particle size (p < 0.05) and reduced the zeta potential of the nanoemulsions. Regarding the color analysis, a higher amount of ZL extract resulted in intense green and yellow color index but tended to decrease lightness of the formulations. For the encapsulation efficiency of 4- terpineol, 10% ZLNE showed the highest encapsulation efficiency of 94.73% . It was in agreement with the antioxidant activity, phenolic content, and flavonoid content showing that 10% ZLNE has significantly higher bioactivity (p < 0.05) than ZLNE 5% and ZLNE 1%, respectively. In conclusion, increasing the content of ZL extract promoted the bioactivities of the nanoemulsions. However, the stability of the nanoemulsion formulation requires further improvement before it can be used in pharmaceutical and cosmetics products.
Keywords
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DOI: 10.14416/j.kmutnb.2024.03.014
ISSN: 2985-2145
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