Extraction of Chitin from Giant Tiger Prawn (Penaeus monodon) Shrimp Shell Using Deep Eutectic Solvents and Citric Acid
Abstract
Chitin, a natural biomass resource, has shown great promise for a wide range of applications because of its high bioactivity. This study evaluated the effectiveness of deep eutectic solvents (DESs) mixed with citric acid as a method for extracting chitin from the shells of giant tiger prawn shrimps (Penaeus monodon). The purity and physicochemical properties of the extracted chitin were compared with those obtained using the traditional chemical extraction method and commercial chitin. The results showed that the highest chitin purity (99.22%) was achieved when choline chloride-glycerol (ChCl–Gl) was used in a 1:2 molar ratio with a citric acid content of 5% w/v (CG2-5%). Additionally, the extracted chitin had a molecular weight of 3.75 × 105 Da and a crystallinity index of 81.34%, which was slightly higher than that of chitin extracted using the conventional method (3.24 × 105 Da and 73.59%). However, there was no significant difference between chitin extracted by CG2-5% and commercial chitin. This suggests that the structure of the biopolymer remained intact following the CG2-5% extraction process. The α-chitins in tiger prawn shrimp shells, as confirmed by Scanning Electron Microscopy (SEM), Fourier Transform Infrared (FTIR) spectroscopy, and X-ray Diffraction Analysis (XRD), are analogous to commercial shrimp chitin. These results, achieved without employing potentially harmful chemicals, demonstrate that CG2-5% can efficiently enhance chitin extraction from diverse raw biomass sources without jeopardizing the polymer's structural stability.
Keywords
[1] P. Ramasamy, N. Subhapradha, V. Shanmugam, and A. Shanmugam, “Extraction, characterization and antioxidant property of chitosan from cuttlebone Sepia kobiensis (Hoyle 1885),” International Journal of Biological Macromolecules, vol. 64, pp. 202–212, Mar. 2014, doi: 10.1016/j.ijbiomac. 2013.12.008.
[2] K. Mohan, T. Muralisankar, R. Jayakumar, and C. Rajeevgandhi, “A study on structural comparisons of α-chitin extracted from marine crustacean shell waste,” Carbohydrate Polymer Technologies and Applications, vol. 2, Dec. 2021, Art. no. 100037, doi: 10.1016/j.carpta.2021.100037.
[3] Z. Li, C. Liu, S. Hong, H. Lian, C. Mei, J. Lee, Q. Wu, M. A. Hubbe, and M. C. Li, “Recent advances in extraction and processing of chitin using deep eutectic solvents,” Chemical Engineering Journal, vol. 446, no. 2, 2022, Art. no. 136953, doi: 10.1016/j.cej.2022.136953.
[4] D. Zhao, W. C. Huang, N. Guo, S. Zhang, C. Xue, and X. Mao, “Two-step separation of chitin from shrimp shells using citric acid and deep eutectic solvents with the assistance of microwave,” Polymers (Basel), vol. 11, no. 3, 2019, Art. no. 409, doi: 10.3390/polym11030409.
[5] F. A. A. Sagheer, M. A. Al-Sughayer, S. Muslim, and M. Z. Elsabee, “Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf,” Carbohydrate Polymers, vol. 77, no. 2, pp. 410–419, Jun. 2009. doi: 10.1016/ j.carbpol.2009.01.032.
[6] M. Maddaloni, I. Vassalini, and I. Alessandri, “Green routes for the development of chitin/ chitosan sustainable hydrogels,” Sustainable Chemistry, vol. 1, no. 3, pp. 325–344, 2020, doi: 10.3390/suschem1030022.
[7] B. K. Park and M. M. Kim, “Applications of chitin and its derivatives in biological medicine,” International Journal of Molecular Sciences, vol. 11, no. 12, pp. 5152–5164, 2010, doi: 10.3390/ ijms11125152.
[8] N. M. Alves and J. F. Mano, “Chitosan derivatives obtained by chemical modifications for biomedical and environmental applications,” International Journal of Biological Macromolecules, vol. 43, no. 5, pp. 401–414, 2008, doi: 10.1016/j.ijbiomac. 2008.09.007.
[9] F. Khoushab and M. Yamabhai, “Chitin research revisited,” Marine Drugs, vol. 8, no. 7, pp. 1988– 2012, 2010, doi: 10.3390/md8071988.
[10] G. A. F. Roberts, Chitin Chemistry. UK: The Macmillan Press LTD, pp. 20–33, 1992, doi: 10.1007/978-1-349-11545-7.
[11] S. W. Kim, Chitin, Chitosan, Oligosacharides, and their Derivaties: Biological Activities and Applications. Florida: CRC Press, pp. 37–42, 2010.
[12] D. Jose, N. Kitiborwornkul, M. Sriariyanun, and K. Keerthi, “A review on chemical treatment methods of lignocellulosic biomass: Recent advances and progress,” Applied Science and Engineering Progess, vol. 15, no. 4, 2022, Art. no. 6210, doi: 10.14416/j.asep.2022.08.001.
[13] J. Zhang, W. R. Xu, and Y. C. Zhang, “Facile production of chitin from shrimp shells using a deep eutectic solvent and acetic acid,” Royal Society of Chemistry Advances, vol. 12, pp. 22631–22638, 2022, doi: 10.1039/d2ra03417d.
[14] S. Areeya, E. J. Panakkal, M. Sriariyanun, T. Kangsadam, A. Tawai, S. Amornraksa, U. W. Hartley, and P. Yasurin, “A review on chemical pretreatment of lignocellulosic biomass for the production of bioproducts: Machanism, challenges, and applications,” Applied Science and Engineering Progess, vol. 16, no. 3, 2023, Art. no. 6767, doi: 10.14416/j.asep.2023.02.008.
[15] P. S. Saravana, T. C. Ho, S.-J. Chae, Y.-J. Cho, J.-S. Park, H.-J Lee, and B.-S. Chun, “Deep eutectic solvent-based extraction and fabrication of chitin films from crustacean waste,” Carbohydrate Polymers, vol. 195, pp. 622–630, 2018, doi: 10.1016/j.carbpol.2018.05.018.
[16] J. Xie, W. Xie, J. Yu, R. Xin, Z. Shi, L. Song, and X. Yang, “Extraction of chitin from shrimp shell by Successive two-step fermentation of Exiguobacterium profundum and Lactobacillus acidophilus,” Frontiers in Microbiology, vol. 12, 2021, Art. no. 677126, doi: 10.3389/fmicb. 2021.677126.
[17] K. Morgan, C. Conway, S. Faherty, and C. Quigley, “A comparative analysis of conventional and deep eutectic solvent (Des)-mediated strategies for the extraction of chitin from marine crustacean shells,” Molecules, vol. 26, no. 24, 2021, Art. no. 7603, doi: 10.3390/molecules26247603.
[18] Y. Wang, Y. Yang, R. Wang, Y. Zhu, P. Yang, Z. Lin, Z. Wang, and W. Cong, “Effectively inhibiting the degradation of chitin during extraction from crustacean waste via a novel deep eutectic solvent aqueous solution,” Process Biochemistry, vol. 121, pp. 142–151, 2022, doi: 10.1016/j.procbio.2022.06.029.
[19] X. Sun, Q. Wei, Y. Yang, Z. Xiao, and X. Ren, “In-depth study on the extraction and mechanism of high-purity chitin based on NADESs method,” Journal of Enviromental Chemical Engineering, vol. 10, no. 1, 2022, Art. no. 106859, doi: 10.1016/j.jece.2021.106859.
[20] Y. Qin, X. Lu, N. Sun, and R. D. Rogers, “Dissolution or extraction of crustacean shells using ionic liquids to obtain high molecular weight purified chitin and direct production of chitin films and fibers,” Green Chemistry, vol. 12, no. 6, pp. 968–997, 2010, doi: 10.1039/c003583a.
[21] N. J. Kruger, “The Bradford Method for protein quantitation” in The Protein Protocols Handbook, W. J. Walker, Eds. New Jersey: Human Press, pp. 11–15, 2002.
[22] M. Feng, X. Lu, J. Zhang, Y. Li, C. Shi, L. Lu, and S. Zhang, “Direct conversion of shrimp shells to: O -acylated chitin with antibacterial and anti-tumor effects by natural deep eutectic solvents,” Green Chemistry, vol. 21, no. 1, pp. 87–98, 2019, doi: 10.1039/c8gc02506a.
[23] W. Wang, S. Bo, S. Li, and W. Qin, “Determination of the Mark-Houwink equation for chitosans with different degrees of deacetylation,” International Journal of Biological Macromolecules, vol. 13, pp. 281–285, 1991.
[24] D. Skarpalezos and A. Detsi, “Deep eutectic solvents as extraction media for valuable flavonoids from natural sources,” Applied Science, vol. 9, no. 9, 2019, Art. no. 4169, doi: 10.3390/ app9194169.
[25] B. Bradić, U. Novak, and B. Likozar, “Crustacean shell bio-refining to chitin by natural deep eutectic solvents,” Green Processing and Synthesis, vol. 9, no. 1, pp. 13–25, 2020, doi: 10.1515/ gps-2020-0002.
[26] M. Bisht, I. P. E. Macário, M. C. Neves, J. L. Pereira, S. Pandey, R. D. Rogers, J. A. P. Coutinho, and S. P. M. Ventura, “Enhanced dissolution of chitin using acidic deep eutectic solvents: A sustainable and simple approach to extract chitin from crayfish shell wastes as alternative feedstocks,” ACS Sustainable Chemistry and Engineering, vol. 9, no. 48, pp. 16073–16081, 2021, doi: 10.1021/acssuschemeng.1c04255.
[27] M.-T. Yen and J.-L. Mau, “Physico-chemical characterization of fungal chitosan from shiitake stipes,” LWT - Food Science and Technology, vol. 40, no. 3, pp. 472–479, 2007, doi: 10.1016/ j.lwt.2006.01.002.
[28] M.-K. Jang, B.-G. Kong, Y.-I. Jeong, C. H. Lee, and J.-W. Nah, “Physicochemical characterization of α-chitin, β-chitin, and γ-chitin separated from natural resources,” Journal of Polymer Science Part A: Polymer Chemistry, vol. 42, no. 14, pp. 3423–3432, Jul. 2004, doi: 10.1002/pola.20176.
[29] O. P. Gbenebor, S. O. Adeosun, G. I. Lawal, S. Jun, and S. A. Olaleye, “Acetylation, crystalline and morphological properties of structural polysaccharide from shrimp exoskeleton,” Engineering Science and Technology, an International Journal, vol. 20, no. 3, pp. 1155– 1165, 2017, doi: 10.1016/j.jestch.2017.05.002.
DOI: 10.14416/j.asep.2023.10.006
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