Optimization of Enzyme-assisted Extraction of Bioactive Peptides from Whiteleg Shrimp (Litopenaeus vannamei) Head Waste using Box-Behnken Design
Abstract
Whiteleg shrimp (Litopeneaus vannamei) processing lines discard huge quantities of head wastes, which are the protein-rich source. Enzymatic hydrolysis has been studied for decades to salvage discarded whiteleg shrimp head waste to produce high-added value products applicable to animal industry. Alcalase 2.5 L, which is widely applied on marine materials, was used to hydrolyze whiteleg shrimp head waste protein and then purified by cold absolute ethanol. Hydrolysis conditions including enzyme/substrate (E/S) ratio, incubation temperature and duration were preliminarily screened (E/S ratio 0–2.5%, temperature 50–70 °C, duration 30–90 min) before conducting optimization using Box-Behnken design. After optimization, collected protein hydrolysate reached an experimental yield of 61.64% on dry basis under the optimal conditions as treated by Alcalase 2.5 L at 0.9% E/S ratio and incubated at 62 °C for 65 min. Protein hydrolysate performed bioactivity including DPPH (2,2-diphenyl-1-picrylhydrazyl) free radical scavenging activity (3.88 ± 0.10 mg Trolox equivalents g dry matter–1), reducing power (19.20 ± 0.52 mg Trolox equivalents g dry matter–1) and α-amylase inhibitory activity (10.74 ± 0.65% inhibition at 50 mg mL–1).
Keywords
[1] N. P. Nirmal, C. Santivarangkna, M. S. Rajput, and S. Benjakul, “Trends in shrimp processing waste utilization: An industrial prospective,” Trends in Food Science & Technology, vol. 103, pp. 20–35, 2020.
[2] Monterey Bay Aquarium Seafood Watch, “Farmed giant tiger prawns in Vietnam,” 2021. [Online]. Available https://www.seafoodwatch. org/our-projects/farmed-shrimp-in-vietnam
[3] G. M. Mathew, D. C. Mathew, R. K. Sukumaran, R. Sindhu, C.- C. Huang, P. Binod, R. Sirohi, S.-H. Kim, and A. Pandey, “Sustainable and eco-friendly strategies for shrimp shell valorization,” Environmental Pollution, vol. 267, 2020, Art. no. 115656.
[4] Y.-S. Cheng, P. Mutrakulcharoen, S. Chuetor, K. Cheenkachorn, P. Tantayotai, E. J. Panakkal, and M. Sriariyanun, “Recent situation and progress in biorefining process of lignocellulosic biomass: Toward green economy,” Applied Science and Engineering Progress, vol. 13, no. 4, pp. 299– 311, 2020, doi: 10.14416/j.asep.2020.08.002.
[5] A. Kannan, N. S. Hettiarachchy, M. Marshall, S. Raghavan, and H. Kristinsson, “Shrimp shell peptide hydrolysates inhibit human cancer cell proliferation,” Journal of the Science of Food and Agriculture, vol. 91, no. 10, pp. 1920–1924, 2011.
[6] T. O. Awosika and R. E. Aluko, “Inhibition of the in vitro activities of α‐amylase, α‐glucosidase and pancreatic lipase by yellow field pea (Pisum sativum L.) protein hydrolysates,” International Journal of Food Science & Technology, vol. 54, no. 6, pp. 2021–2034, 2019.
[7] G. Yuan, W. Li, Y. Pan, C. Wang, and H. Chen, “Shrimp shell wastes: Optimization of peptide hydrolysis and peptide inhibition of α-amylase,” Food Bioscience, vol. 25, pp. 52–60, 2018.
[8] V. G. Tacias-Pascacio, R. Morellon-Sterling, E.-H. Siar, O. Tavano, A. Berenguer-Murcia, and R. Fernandez-Lafuente, “Use of Alcalase in the production of bioactive peptides: A review,” International Journal of Biological Macromolecules, vol. 165, pp. 2143–2196, 2020.
[9] S. S. Dey and K. C. Dora, “Optimization of the production of shrimp waste protein hydrolysate using microbial proteases adopting response surface methodology,” Journal of Food Science and Technology, vol. 51, no. 1, pp. 16–24, 2014.
[10] R. Sowmya, T. Ravikumar, R. Vivek, K. Rathinaraj, and N. Sachindra, “Optimization of enzymatic hydrolysis of shrimp waste for recovery of antioxidant activity rich protein isolate,” Journal of Food Science and Technology, vol. 51, no. 11, pp. 3199–3207, 2014.
[11] T.-B. Zou, T.-P. He, H.-B. Li, H.-W. Tang, and E.-Q. Xia, “The structure-activity relationship of the antioxidant peptides from natural proteins,” Molecules, vol. 21, no. 1, p. 72, 2016.
[12] I. D. Nwachukwu and R. E. Aluko, “Structural and functional properties of food protein‐derived antioxidant peptides,” Journal of Food Biochemistry, vol. 43, no. 1, p. e12761, 2019.
[13] H. He, X. Chen, C. Sun, Y. Zhang, and P. Gao, “Preparation and functional evaluation of oligopeptide-enriched hydrolysate from shrimp (Acetes chinensis) treated with crude protease from Bacillus sp. SM98011,” Bioresource Technology, vol. 97, no. 3, pp. 385–390, 2006.
[14] F. Guerard, M. T. Sumaya-Martinez, D. Laroque, A. Chabeaud, and L. Dufossé, “Optimization of free radical scavenging activity by response surface methodology in the hydrolysis of shrimp processing discards,” Process Biochemistry, vol. 42, no. 11, pp. 1486–1491, 2007.
[15] S. Kalambura, T. Krička, D. Kiš, S. Guberac, D. Kozak, and A. Stoić, “High-risk bio-waste processing by alkaline hydrolysis and isolation of amino acids,” Tehnički Vjesnik, vol. 23, no. 6, pp. 1771–1776, 2016.
[16] H. G. Kristinsson and B. A. Rasco, “Fish protein hydrolysates: Production, biochemical, and functional properties,” Critical Reviews in Food Science and Nutrition, vol. 40, no. 1, pp. 43–81, 2000.
[17] A. Ghaly, V. Ramakrishnan, M. Brooks, S. Budge, and D. Dave, “Fish processing wastes as a potential source of proteins, amino acids and oils: A critical review,” Journal of Microbial and Biochemical Technology, vol. 5, no. 4, pp. 107–129, 2013.
[18] H. D. De Holanda and F. M. Netto, “Recovery of components from shrimp (Xiphopenaeus kroyeri) processing waste by enzymatic hydrolysis,” Journal of Food Science, vol. 71, no. 5, pp. C298–C303, 2006.
[19] T. T. V. Ha, V. M. Nguyen, and T. T. Tran, “Optimization of protein hydrolysis conditions from shrimp head meat (Litopenaeus vannamei) using commercial alcalase and flavourzyme enzymes,” Can Tho University Journal of Science, vol. 54, pp. 16–25, 2018.
[20] S. See, L. Hoo, and A. Babji, “Optimization of enzymatic hydrolysis of Salmon (Salmo salar) skin by Alcalase,” International Food Research Journal, vol. 18, no. 4, p. 1359, 2011.
[21] N. Wisuthiphaet, S. Klinchan, and S. Kongruang, “Fish protein hydrolysate production by acid and enzymatic hydrolysis,” Applied Science and Engineering Progress, vol. 9, no. 4, 2016, doi: 10.14416/j.ijast.2016.11.004.
[22] H. K. Mæhre, L. Dalheim, G. K. Edvinsen, E. O. Elvevoll, and I.-J. Jensen, “Protein determination— method matters,” Foods, vol. 7, no. 1, p. 5, 2018.
[23] O. H. Lowry, “Protein measurement with the Folin phenol reagent,” Journal of Biological Chemistry, vol. 193, pp. 265–275, 1951.
[24] J. Zhao, G. Huang, and J. Jiang, “Purification and characterization of a new DPPH radical scavenging peptide from shrimp processing by-products hydrolysate,” Journal of Aquatic Food Product Technology, vol. 22, no. 3, pp. 281–289, 2013.
[25] M. Irshad, M. Zafaryab, M. Singh, and M. Rizvi, “Comparative analysis of the antioxidant activity of Cassia fistula extracts,” International Journal of Medicinal Chemistry, vol. 2012, 2012, Art. no. 157125.
[26] V. M. Nguyen, N. T. D. Nguyen, and T. T. Tran, “Factors affecting the drying process of shrimp powder from black tiger shrimp (Penaeus monodon) head meat,” in Proceedings of the 2nd AFSSA Conference on Food Safety and Food Security, pp. 83–88, 2014.
[27] H. Ibrahim, M. Salama, and H. El‐Banna, “Shrimp's waste: Chemical composition, nutritional value and utilization,” Food/Nahrung, vol. 43, no. 6, pp. 418–423, 1999.
[28] B. Narayan, S. C. Hathwar, and D. Hemavathi, “Lipid class and fatty acid composition of meat and nonmeat components of selected seafoods,” Indian Journal of Fisheries, vol. 59, no. 1, pp. 133– 139, 2012.
[29] A. D. S. Abreu, M. M. De Souza, M. Da Rocha, W. F. Wasielesky, and C. Prentice, “Functional properties of white shrimp (Litopenaeus v annamei) by-Products protein recovered by isoelectric solubilization/precipitation,” Journal of Aquatic Food Product Technology, vol. 28, no. 6, pp. 649–657, 2019.
[30] S. Ravichandran, G. Rameshkumar, and A. R. Prince, “Biochemical composition of shell and flesh of the Indian white shrimp Penaeus indicus (H. milne Edwards 1837),” American-Eurasian Journal of Scientific Research, vol. 4, no. 3, pp. 191–194, 2009.
[31] A. Mittal, A. Singh, R. E. Aluko, and S. Benjakul, “Pacific white shrimp (Litopenaeus vannamei) shell chitosan and the conjugate with epigallocatechin gallate: Antioxidative and antimicrobial activities,” Journal of Food Biochemistry, vol. 45, no. 1, p. e13569, 2021.
[32] S. Sinthusamran, S. Benjakul, K. Kijroongrojana, T. Prodpran, and H. Kishimura, “Protein hydrolysates from Pacific white shrimp cephalothorax manufactured with different processes: Compositions, characteristics and antioxidative activity,” Waste and Biomass Valorization, vol. 11, no. 5, pp. 1657–1670, 2020.
[33] S. Saidi, M.-P. Belleville, A. Deratani, and R. B. Amar, “Optimization of peptide production by enzymatic hydrolysis of tuna dark muscle by-product using commercial proteases,” African Journal of Biotechnology, vol. 12, no. 13, pp. 1533–1547, 2013.
[34] F. Guérard, L. Guimas, and A. Binet, “Production of tuna waste hydrolysates by a commercial neutral protease preparation,” Journal of Molecular Catalysis B: Enzymatic, vol. 19, pp. 489–498, 2002.
[35] M. A. Vieira, D. D. Oliveira, and L. E. Kurozawa, “Production of peptides with radical scavenging activity and recovery of total carotenoids using enzymatic protein hydrolysis of shrimp waste,” Journal of Food Biochemistry, vol. 40, no. 4, pp. 517–525, 2016.
[36] M. A. Khesal, A. Sharifan, E. Hoseini, and A. Ghavami, “Optimization of enzymatic hydrolysis conditions of Caspian kutum (Rutilus frisii kutum) ˮ By-product for Production of Bioactive peptides with antioxidative properties,” International Journal of Peptide Research and Therapeutics, vol. 26, no. 4, pp. 1829–1838, 2020.
[37] O. Awuor, M. E. Kirwa, M. Betty, and M. Jackim, “Optimization of Alcalase hydrolysis conditions for production of Dagaa (Rastrineobola argentea) Protein hydrolysate with antioxidative properties,” Industrial Chemistry, vol. 3, 2017, Art. no. 122.
[38] H. Rasli and N. Sarbon, “Optimization of enzymatic hydrolysis conditions and characterization of Shortfin scad (Decapterus macrosoma) skin gelatin hydrolysate using response surface methodology,” International Food Research Journal, vol. 25, no. 4, pp. 1541–1549, 2018.
[39] I. Normah, B. Jamilah, N. Saari, and B. C. M. Yaakob, “Optimization of hydrolysis conditions for the production of threadfin bream (Nemipterus japonicus) hydrolysate by Alcalase,” Journal of Muscle Foods, vol. 16, no. 2, pp. 87–102, 2005.
[40] N. Ahmadifard, J. H. C. Murueta, A. Abedian- Kenari, A. Motamedzadegan, and H. Jamali, “Comparison the effect of three commercial enzymes for enzymatic hydrolysis of two substrates (rice bran protein concentrate and soy-been protein) with SDS-PAGE,” Journal of Food Science and Technology, vol. 53, no. 2, pp. 1279–1284, 2016.
[41] J. Gunasekaran, N. Kannuchamy, S. Kannaiyan, R. Chakraborti, and V. Gudipati, “Protein hydrolysates from shrimp (Metapenaeus dobsoni) head waste: optimization of extraction conditions by response surface methodology,” Journal of Aquatic Food Product Technology, vol. 24, no. 5, pp. 429–442, 2015.
[42] P. Borrajo, M. Pateiro, M. Gagaoua, D. Franco, W. Zhang, and J. M. Lorenzo, “Evaluation of the antioxidant and antimicrobial activities of porcine liver protein hydrolysates obtained using alcalase, bromelain, and papain,” Applied Sciences, vol. 10, no. 7, p. 2290, 2020.
[43] S. Athira, B. Mann, P. Saini, R. Sharma, R. Kumar, and A. K. Singh, “Production and characterisation of whey protein hydrolysate having antioxidant activity from cheese whey,” Journal of the Science of Food and Agriculture, vol. 95, no. 14, pp. 2908–2915, 2015.
[44] P. Ambigaipalan, A. S. Al-Khalifa, and F. Shahidi, “Antioxidant and angiotensin I converting enzyme (ACE) inhibitory activities of date seed protein hydrolysates prepared using Alcalase, Flavourzyme and Thermolysin,” Journal of Functional Foods, vol. 18, pp. 1125–1137, 2015.
DOI: 10.14416/j.asep.2023.09.002
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